Phage displayed domain antibody mimics for pyrethroid and its application in immunoassay

Yanyan Zhao, Ying Liang, Yuan Liu, Sicong Tu, Shengming Zhao, Xianjin Liu, Kang Tu

Abstract


Anti-idiotypic antibodies which can mimic antigens have many potential applications in the immunoassay. This research used the monoclonal antibody of the conserved chemical region of pyrethroids, 3-phenoxybenzoic acid (PBA), and the domain antibody library to develop an environmentally-friendly immunoassay for the detection of pyrethroids. The domain antibodies (A8, B8, and C6) which bound to anti-PBA monoclonal antibody (MAb) were isolated from a naive phage display human domain antibody library. This domain antibody is cloneable, pyrethroid-free, and applicable as a competitive mimetic antigen in the immunoassay. The best immunoassay was achieved using A8, resulting in IC50 of 0.714 μg/mL for PBA, 1.775 μg/mL for Cypermethrin, 1.624 μg/mL for β-cypermethrin, 3.675 μg/mL for Fenvalerate, and 4.895 μg/mL for Flucythrinate. This way of selecting anti-idiotypic antibodies to detect pyrethroids could provide potential applications in developing immunoassays for identifying various chemical contaminants in food.
Keywords: domain antibody, enzyme-linked immunosorbent assay, phenoxybenzoic acid, pyrethroid
DOI: 10.25165/j.ijabe.20201302.5492

Citation: Zhao Y Y, Liang Y, Liu Y, Tu S C, Zhao S M, Liu X J, et al. Phage displayed domain antibody mimics for pyrethroid and its application in immunoassay. Int J Agric & Biol Eng, 2020; 13(2): 235–240.

Keywords


domain antibody, enzyme-linked immunosorbent assay, phenoxybenzoic acid, pyrethroid

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References


Ellis D I, Brewster V L, Dunn W B, Allwood J W, Golovanov A P,

Goodacre R. Fingerprinting food: current technologies for the detection of food adulteration and contamination. Chemical Society Reviews, 2012; 41: 5706–5727.

Campàs M, Garibo D, Prieto-Simón B. Novel nanobiotechnological concepts in electrochemical biosensors for the analysis of toxins. Analyst, 2012; 137: 1055–1067.

Cháfer-Pericás C, Maquieira Á, Puchades R. Fast screening methods to detect antibiotic residues in food samples. Trac Trends in Analytical Chemistry, 2010; 29(9): 1038–1049.

Wang Y, Wang Z, Jiang H, Xia X, Shen J, Ding S. Development of a monoclonal antibody-based enzyme-linked immunosorbent assay for the analysis of diclazuril in chicken tissues. Food Analytical Methods, 2013; 6(6): 1685–1692.

Song S, Suryoprabowo S, Liu L, Kuang H, Xu L, Ma W, et al. Development of monoclonal antibody-based colloidal gold immunochromatographic assay for analysis of halofuginone in milk. Food and Agricultural Immunology, 2019; 30(1): 112–122.

Xu Y, Chen B, He Q H, Qiu Y L, Liu X, He Z Y, et al. New approach for development of sensitive and environmentally friendly immunoassay for Mycotoxin Fumonisin B1 based on using Peptide-MBP fusion protein as substitute for coating antigen. Analytical Chemistry, 2014; 86: 8433–8440.

Xu Y, He Z, He Q, Qiu Y, Chen B, Chen J, et al. Use of cloneable peptide–mbp fusion protein as a mimetic coating antigen in the standardized immunoassay for Mycotoxin Ochratoxin A. Journal of Agricultural and Food Chemistry, 2014; 62: 8830–8836.

Shestowsky W S, Holmes C, Hu T, Marr J, Wright J, Chin J, et al. An anti-okadaic acid-anti-idiotypic antibody bearing an internal image of okadaic acid inhibits protein phosphatase PP1 and PP2A catalytic activity. Biochemical and Biophysical Research Communications, 1993; 192: 302–310.

Chanh T C, Rappocciolo G, Hewetson J F. Monoclonal anti-idiotype induces protection against the cytotoxicity of the trichothecene mycotoxin T-2. Journal of Immunology, 1990; 144: 4721–4728.

Yu F Y, Chu F S. Production and characterization of a monoclonal anti-anti-idiotype antibody against fumonisin B1. Journal of Agricultural and Food Chemistry, 1999; 47: 4815–4820.

Guan D, Li P, Cui Y, Zhang Q, Zhang W. A competitive immunoassay with a surrogate calibrator curve for aflatoxin M 1 in milk. Analytica Chimica Acta, 2011; 703: 64–69.

Smith G P. Filamentous fusion phage: Novel expression vectors that display cloned antigens on the virion surface. Science, 1985; 228: 1315–1317.

Wang Y, Wang H, Li P, Zhang Q, Kim H J, Gee S J, et al. Phage-displayed peptide that mimics aflatoxins and its application in immunoassay. Journal of Agricultural and Food Chemistry, 2013; 61: 2426–2433.

Wang Y, Li P, Majkova Z, Bever C R, Kim H J, Zhang Q, et al. Isolation of alpaca anti-idiotypic heavy-chain single-domain antibody for the aflatoxin immunoassay. Analytical Chemistry, 2013; 85: 8298–8303.

Liu R, Yu Z, He Q, Xu Y. An immunoassay for ochratoxin A without the mycotoxin. Food Control, 2007; 18: 872–877.

Nejad S M, Veldhuis S L, Richard G, Hall J C. Selection of single chain variable fragment (scFv) antibodies from a hyperimmunized phage display library for the detection of the antibiotic monensin. Journal of Immunological Methods, 2010; 360: 103–118.

González-Techera A, Zon M A, Molina P G, Fernández H, González-Sapienza G, Arévalo F J. Development of a highly sensitive noncompetitive electrochemical immunosensor for the detection of atrazine by phage anti-immunocomplex assay. Biosensors and Bioelectronics, 2015; 64: 650–656.

Yin W, Hua X, Liu X, Shi H, Gee S J, Wang M, Hammock B D. Development of an enzyme-linked immunosorbent assay for thiacloprid in soil and agro-products with phage-displayed peptide. Analytical Biochemistry, 2015; 481: 27–32.

Yuan Q, Pestka J J, Hespenheide B M, Kuhn L A, Linz J E, Hart L P. Identification of mimotope peptides which bind to the mycotoxin deoxynivalenol-specific monoclonal antibody. Appl. Environmental Microbiology, 1999; 65: 3279–3286.

He Q H, Xu Y, Huang Y H, Liu R R, Huang Z B, Li Y P. Phage-displayed peptides that mimic zearalenone and its application in immunoassay. Food Chemistry, 2011; 126: 1312–1315.

Li Y, Sun Y, Beier R, Lei H, Gee S, Hammock B, et al. Immunochemical techniques for multianalyte analysis of chemical residues in food and the environment: A review. Trac-Trends in Analytical Chemistry, 2017; 88: 25–40.

Li C, Luo X, Li Y, Yang H, Liang X, Wen K, et al. A class-selective immunoassay for sulfonamides residue detection in milk using a superior polyclonal antibody with broad specificity and highly uniform affinity. Molecules, 2019; 24(3): 443.

Wen M T, Liu Y, Yan S, Zhang X, Wang H, Liu X J. Production and identification of broad spectrum monoclonal antibody against a group of pyrethroid insecticides. Chinese Journal of Analytical Chemistry, 2014, 42(9): 1245–1251. (in Chinese)

Lee C M Y, Niccolo I, Frederic S, Daniel C. Selection of human antibody fragments by phage display. Nature Protocols, 2007; 2: 3001–3008.

Zhang Q, Zhang W, Wang X. Immunoassay development for the class-specific assay for types I and II pyrethroid insecticides in water samples. Molecules, 2010; 15: 164–177.

Delaunay-Bertoncini N, Pichon V, Hennion M C. Experimental comparison of three monoclonal antibodies for the class-selective immunoextraction of triazines: Correlation with molecular modeling and principal component analysis studies. Journal of Chromatography A, 2003; 999: 3–15.

Ahn K C, Kim H-J, McCoy M R, Gee S J, Hammock B D. Immunoassays and biosensors for monitoring environmental and human exposure to pyrethroid insecticides. Journal of Agricultural and Food Chemistry, 2010; 59: 2792–2802.

Vanderlaan M, Stanker L, Watkins B. Immunoassays for trace chemical analysis: monitoring toxic chemicals in humans, food, and the environment; American Chemical Society; Washington, DC, 1991; Paper No. 2–13.




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