Optimization of fermentation factors to enhance rice straw degradation ability using a microbial consortium LZF-12

Guoxiang Zheng, Chenyang Zhou, Ting Yin, Zhaoxin Lu, Shuang Ai

Abstract


Biological pretreatment has broad application prospects in agricultural waste treatment because of its economic, environmental protection and energy saving characteristics. In this study, a microbial consortium LZF-12 was applied as a biological pretreatment to degrade rice straw. Batch experiments were performed to hydrolysis conditions by using the method of Box-Behnken factorial design (BBD). The results showed that the model multiple correlation coefficient R2 was 0.9816, and the effects of three factors on the degradation of rice straw of LZF-12 as descending order were the initial rice content, chicken manure content, and initial pH. The interaction between straw concentration, chicken manure concentration, and initial pH had significant effects on the degradation of a microbial consortium. Under the optimum conditions of 0.86% rice straw, 0.5% chicken manure and the initial pH of 7.0, the degradation rate of rice straw reached 72.4%. There is only small difference of 0.55% between the experimental value and predicted value from BBD model. Therefore, it is feasible for the established model due to the consistent results between the prediction and experimental value. The microbial consortium LZF-12 has high cellulase enzyme activities and degradation ability over a wide range of temperature and pH, indicating it has a good development potential and application prospects in waste biodegradation and biomass energy production.
Keywords: microbial consortium, biological pretreatment, biodegradation, rice straw, optimization
DOI: 10.25165/j.ijabe.20191203.4638

Citation: Zheng G X, Zhou C Y, Yin T, Lu Z X, Ai S. Optimization of fermentation factors to enhance rice straw degradation ability using a microbial consortium LZF-12. Int J Agric & Biol Eng, 2019; 12(3): 168–173.

Keywords


microbial consortium, biological pretreatment, biodegradation, rice straw, optimization

Full Text:

PDF

References


Kang P, Gary M, Zhang X L, Wang T P, Tan Z F, Agarwal J. Systematic comparison of hydrogen production from fossil fuels and biomass resources. Int J Agric & Biol Eng, 2017; 11: 192–200.

Souichiro K, Shin H, Zong J C, Masaharu I, Yasuo I. Effective cellulose degradation by a mixed-culture system composed of a cellulolytic clostridium and aerobic non-cellulolytic bacteria. FEMS Microbiology Ecology, 2004; 51: 133–142.

Zhong Y T. Research on screening of rice straw degradation microbial system and its fermentation characteristics. Harbin, Northeast Agricultural University, 2011. (in Chinese)

Souichiro K, Shin H, Zong J C, Masaharu I, Yasuo I. Stable coexistence of five bacterial strains as a cellulose-degrading community. Applied and Environmental Microbiology, 2005; pp: 7099–7106.

Soundar S, Chandra T S. Cellulose degradation by a mixed bacterial culture. Ind Microbial, 1987; 2: 257–265.

Gu F, Wang W, Jing L, Jin Y. Effects of green liquor pretreatment on the chemical composition and enzymatic digestibility of rice straw. Bioresource Technology, 2013; 149: 375–382.

Du W, Yu H, Song L, Zhang J, Weng, C, Ma F, et al. The promoting effect of byproducts from Irpex lacteus on subsequent enzymatic hydrolysis of bio-pretreated cornstalks. Biotechnology for Biofuels, 2011; 11(4): 37–48.

Haruta S, Cui Z, Huang Z, Li M, Ishii M, Igarashi Y. Construction of a stable microbial consortium with high cellulose-degradation ability. Applied Microbiology and Biotechnology, 2002; 59(4-5): 529–534.

Han X, Song W, Liu G, L, Z, Yang P, Qu Y. Improving cellulase productivity of Penicillium oxalicum RE-10 by repeated fed-batch fermentation strategy. Bioresource Technology, 2017; 227: 155–163.

Jung D U, Yoo H Y, Kim S B, Lee J H, Park C. Optimization of medium composition for enhanced cellulase production by mutant Penicillium brasilianum KUEB15 using statistical method. J. Ind. Eng. Chem, 2015; 25: 145–150.

Matkar K, Chapla D, Divecha J, Nighojkar A, Madamwar D. Production of cellulase by a newly isolated strain of Aspergillus sydowii and its optimization under submerged fermentation. Int. Biodeterior. Biodegrad, 2013; 78: 24–33.

Ortiz G E, Guitart M E, Cavalitto S F, Alberto E O, Fernandez-Lahore M. Characterization, optimization, and scale-up of cellulases production by Trichoderma reesei cbs 836.91 in solid-state fermentation using agroindustrial products. Bioprocess. Biosyst. Eng, 2015; 38: 2117–2128.

Guo P, Zhu W B, Wang H, et al. Functional characteristics and diversity of a novel lignocelluloses degrading composite microbial consortium with high xylanase activity. Journal of Microbiology and Biotechnology; 2010; 20(2): 254-264.

Zhong Y T, Li W Z, Zheng G X, Screening of rice straw degradation microbial consortium and its growth characteristics. Journal of Northeast Agricultural University. 2011; 42(8): 56-61..

Amnuaycheewa P, Hengaroonprasan R, Rattanaporn K, Kirdponpattara S, Cheenkachorn K. Enhancing enzymatic hydrolysis and biogas production from rice straw by pretreatment with organic acids. IndCrops Prod, 2016; 87: 247–254.

Song Z, Sun X, Yang G, Yan Z, Yuan Y, Li D, et al. Effect of NaOH pretreatment on methane yield of corn straw at different temperatures by anaerobic digestion. Journal of Chemical Industry and Engineering, 2014; 65(5): 1876–1882. (in Chinese)

Liu S. Characteristic and flora development of a microbial consortium for degradation of lignocelluloses. Harbin, Northeast Agricultural University, 2009. (in Chinese)

Chen L, Luo J, Dong B, Song C, Wan X, Qi Y, et al. Pretreatment with composite microbe and NaOH to improve anaerobic performance of corn straw. Transactions of the CSAE, 2013; 29(7): 185–190. (in Chinese)

Yin L L, Liu S, Wang M Y. Formation process and properties of hydrogen-producing granular sludge in UASB reactor. Int J Agric & Biol Eng, 2018; 9: 224–229.

Mackulak T, Prousek J, Svorc L. Increase of biogas production from pretreated hay and leaves using wood-rotting fungi. Chemical Papers, 2012; 66(7): 649–653.

Kuijk S, Sonnenberg A, Baars J, Hendriks W, Cone J. Fungal treated lignocellulosic biomass as ruminant feed ingredient: A review. Biotechnol Adv, 2015; 33: 191–192.

Zidan A S, Sammour O A, Hammad M A, Megrab N A, Habib M J, Khan M A. Quality by design: understanding the formulation variables of a cyclosporine a self-nanoemulsified drug delivery systems by Box-Behnken design and desirability function. International Journal of Pharmaceutics, 2007; 332: 55.

Chandra R, Takeuchi H, Hasegawa T, Kumar R. Improving biodegradability and biogas production of wheat straw substrates using sodium hydroxide and hydrothermal pretreatments. Energy, 2012; 43(1): 273–282.

Liu S, Wang C Y, Yin L L, Luo L N. Optimization of hydrogen production from agricultural wastes using mixture design. Int J Agric & Biol Eng, 2017; 10(3): 246–254.




Copyright (c) 2019 International Journal of Agricultural and Biological Engineering



2023-2026 Copyright IJABE Editing and Publishing Office