Screening and performance of L-14, a novel, highly efficient and low temperature-resistant cellulose-degrading strain

Guoxiang Zheng, Zhaoxin Lu, Jian Li, Shuang Ai

Abstract


In view of the low bioconversion efficiency of agricultural biomass waste in low-temperature environments in winter, a low-temperature-resistant cellulose-degrading strain, L-14, was successfully screened by restrictive cultures from humus-rich soil in the Daqing Zhalong wetland region. According to morphological observations and 18S rDNA sequence analysis, the cellulose-degrading strain L-14 was identified as a Neurospora sp, belonging to fungus. Different parameters, such as temperature, initial pH, carbon, nitrogen and lecithin, were optimized using a single-factor experiment and a response surface methodology (RSM). When the temperature was 16°C, the optimal conditions for enzyme production were an initial pH 8.20, 10.45 g/L of bran, 5.28 g/L of yeast powder, and 4.25 g/L of lecithin. The carboxymethyl cellulase (CMCase) activity of strain L-14 was 63.598 IU/mL. Strain L-14 had a high level of cellulose degradation activity, excellent resistance to low temperatures and environmental adaptability, indicating its good application prospects in substrates pretreatment of biogas engineering.
Keywords: low-temperature-resistant, Neurospora sp., 18S rDNA sequence, carboxymethyl cellulase, response surface methodology
DOI: 10.25165/j.ijabe.20201301.5128

Citation: Zheng G X, Lu Z X, Li J, Ai S, Sun Y. Screening and performance of L-14, a novel, highly efficient and low temperature-resistant cellulose-degrading strain. Int J Agric & Biol Eng, 2020; 13(1): 247–254.

Keywords


low-temperature-resistant, Neurospora sp., 18S rDNA sequence, carboxymethyl cellulase, response surface methodology

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References


Connell L, Redman R, Craig S, Scorzetti G, Iszard M, Rodriguez R. Diversity of soil yeasts isolated from south victoria land, antarctica. Microbial Ecology, 2008; 56(3): 448–459.

Kang P, Morrow G, Zhang X L, Wang T P, Tan Z F, Agarwal J. Systematic comparison of hydrogen production from fossil fuels and biomass resources. Int J Agric & Biol Eng, 2017; 10(6): 192–200.

Wang Q, Li S N, Li H Y, Lei B S, Chen Y, Zhang L J, et al. Screening and identification on capable of degrading cellulose of produce spore cellulose decomposing bacteria xn-13. Chinese Agricultural Science Bulletin, 2009; 25(11): 180–185. (in Chinese)

Zhao G, Ma F, Wei L, Cai H, Wang Z. Research and prospects of low temperature biogas digestion technology in North. Journal of Harbin Institute of Technology, 2011; 43(6): 29–33. (in Chinese)

Saloheimo M. Swollenin, a trichoderma reesei protein with sequence similarity to the plant expansins, exhibits disruption activity on cellulosic materials. Eur. J. Biochem., 2002; 269(17): 4202–4211.

Hamelinck C N, Hooijdonk G., Faaij A P C. Ethanol from lignocellulosic biomass: techno-economic performance in short-, middle- and long-term. Biomass Bioenerg, 2005; 28: 384–410.

Zhang X F, Yao T D, Tian L D, Xu S J, An L Z. Phylogenetic and physiological diversity of bacteria isolated from puruogangri ice core. Microbial Ecology, 2008; 55(3): 476–488.

Feller U. Moderately high temperatures inhibit ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) activase-mediated activation of rubisco. Plant Physiology, 1998; 116(2): 539–546.

Jarvinen K T, Melin E S, Puhakka J A. High-rate bioremediation of chlorophenol-contaminated groundwater at low temperatures. Environmental Science & Technology, 1994; 28(13): 2387–2392.

Li X M, Jia B X. Isolation and identification of an equol-producing bacterial strain from vegetarian intestinal tract. Food Science, 2014; 35(3): 153–156. (in Chinese)

Li C Y, Xu C H, Pan J B. Isolation of a Chlorimuron-Ethyl Degradation Bacterium and Its Bioremediation in Contamination Soil. International Conference on Bioinformatics & Biomedical Engineering. IEEE. 2011. doi: 10.1109/icbbe.2011.5780302

Kumar S, Nei M, Dudley J, Tamura K. Mega: a biologist-centric software for evolutionary analysis of dna and protein sequences. Briefings in Bioinformatics, 2008; 9(4): 299–306.

Zhao Z Y, Huang Q F, Wang Q. Research progress on the degradation of deca-bdes. Research of Environmental Sciences, 2014; 27(5): 554–559.

Teng G S, Liu Y, Wu L D, Fan C Y, Wang J Z, Guan B, et al. Optimization of l-lysine medium by response surface methodology. Food & Machinery, 2015; 31(5): 256–260. (in Chinese)

Zhang W, Zhang Q, Ruan X Y, Wang X Z, Kong H N, Lin Y. Optimization of enzymatic hydrolysis of wheat straw by using response surface methodology. Biomass Chemical Engineering, 2015; 49(2): 39–46. (in Chinese)

Liu S, Fan B Q. Screening of a straw cellulose-degrading fungi qsh3-3 and study on its characteristics of cellulase production. Plant Nutrition & Fertilizer Science, 2012; 18(1): 218–226.

Zhang C M. Screening of bacteria decomposing low-temperature cellulose and the application of composite microbial inoculants in straw returning to field. MS dissertation, Nanjing Agriculture University, 2014. (in Chinese)

Chen L. Breeding and fermentation medium optimization of cold-active cellulase strain CNY086(I). Microbiology, 2009; 36(10): 1547–1552. (in Chinese)




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