High hydrostatic pressure as a novel strategy for the simultaneous preservation and enhancement of phenylethanol glycosides in fresh-cut Cistanche deserticola

Authors

  • Terigen Bao 1. College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China; 2. College of Mongolian Medicine, Inner Mongolia Medical University, Hohhot 010000, China
  • Qingxin Li 1. College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China
  • Jie Gao 1. College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China
  • Tungalag Dong 1. College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China; 3. China-Mongolia Biomacromolecule Application “Belt and Road” Joint Laboratory, Hohhot 010018, China
  • Eerdunbayaer 1. College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China; 3. China-Mongolia Biomacromolecule Application “Belt and Road” Joint Laboratory, Hohhot 010018, China
  • Lin Song 2. College of Mongolian Medicine, Inner Mongolia Medical University, Hohhot 010000, China
  • Xueyan Yun 3. China-Mongolia Biomacromolecule Application “Belt and Road” Joint Laboratory, Hohhot 010018, China
  • Peifang Cheng 1. College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China

Abstract

This study introduces high hydrostatic pressure (HHP) as an innovative non-thermal technique to simultaneously enhance preservation and extract phenylethanol glycosides, the primary bioactive compounds in fresh-cut Cistanche deserticola. Treatments ranging from 100 to 300 MPa significantly prolonged microbial shelf-life, improved tissue integrity, and increased phenylethanol glycoside yields by up to 34.17%, with optimal accumulation occurring between 9 and 12 d of storage. Microstructural analysis indicated that HHP induced controlled permeability of cell walls, facilitating the release of bioactive compounds without compromising cellular viability. Additionally, HHP stimulated crucial physiological responses such as the activation of phenylalanine ammonia-lyase and enhanced energy metabolism, thereby promoting the biosynthesis and accumulation of the target glycosides. These results illustrate that moderate HHP treatment effectively balances microbial safety, structural quality, and bioactive enhancement, providing a sustainable bioprocessing approach to enhance the value of medicinal plant-based products.      

Keywords: Cistanche deserticola, high hydrostatic pressure, non-thermal preservation, phenylethanol glycosides, microstructural alteration

DOI: 10.25165/j.ijabe.20261902.10365

 

Citation: Bao T R G, Li Q X, Gao J, Dong T, Eerdunbayaer, Song L, et al. High hydrostatic pressure as a novel strategy for simultaneous preservation and enhancement of phenylethanol glycosides in fresh-cut Cistanche deserticola. Int J Agric & Biol Eng, 2026; 19(2): 310–319.

References

[1] Liu J J, Wang Y Y, Li Q Y, Liu T, Liu X, Zhang H, et al. Phenylethanoid glycosides derived from Cistanche deserticola promote neurological functions and the proliferation of neural stem cells for improving ischemic stroke. Biomedicine & Pharmacotherapy, 2023; 167: 115507.

[2] Zhang S A, Ma Y W, Chen J, Yu M L, Zhao Q H, Jing B, et al. Chemical composition, pharmacological effects, and parasitic mechanisms of Cistanche deserticola: An update. Phytomedicine, 2024; 132: 155808.

[3] Zhou S Q, Feng D, Zhou Y X, Zhao J, Zhao J Y, Guo Y, et al. HS-GC-IMS detection of volatile organic compounds in cistanche powders under different treatment methods. LWT-Food Science and Technology, 2022; 165: 113730.

[4] Jin L N, Zhang Z, Song L, Zhao Y Y, Shi J L, Zhang Y M, et al. Effect of sodium alginate composite coating treatment on preservation of fresh-cut Cistanche desertica. Food and Fermentation Industries, 2025; 51（21）: 330–337. （in Chinese）

[5] Ai Z P, Xie Y K, Li X Y, Zhu G F, Peng Z K, Liu Y H, et al. Mechanism of freezing treatment to improve the efficiency of hot air impingement drying of steamed Cistanche deserticola and the quality attribute of the dried product. Industrial Crops and Products, 2023; 195: 116472.

[6] Huang J, Peng Y, Ji Z F, Guo L M, Hao J X, Zhang Z T, et al. Drying characteristics of cistanche deserticola with different pretreatment and degradation mechanism of phenylethanoid glycosides during drying. Industrial Crops and Products, 2023; 204: 117339.

[7] Jao W C, Wu S J. Influence of high hydrostatic pressure treatment on the soluble dietary fiber content and physicochemical characteristics of edamame. LWT-Food Science and Technology, 2024; 202: 116294.

[8] Tian X Z, Lv Y H, Zhao L, Wang Y T, Liao X J. Insight into the mechanism of high hydrostatic pressure effect on inhibitory efficiency of three natural inhibitors on polyphenol oxidase. Food Chemistry, 2024; 457: 140118.

[9] Singh S V, Singh R, Singh A, et al. A review on green pressure processing of fruit juices using microfluidization: Quality, safety and preservation. Applied Food Research, 2022; 2（2）: 100235.

[10] Zhang W J, Shen Y X, Li Z D, Xie X, Gong E S, Tian J L, et al. Effects of high hydrostatic pressure and thermal processing on anthocyanin content, polyphenol oxidase and β-glucosidase activities, color, and antioxidant activities of blueberry （Vaccinium Spp.） puree. Food Chemistry, 2021; 342: 128564.

[11] Rode T M, Hovda M B. High pressure processing extends the shelf life of fresh salmon, cod and mackerel. Food Control, 2016; 70: 242–248.

[12] Ramírez R, Delgado J, Rocha-Pimienta J, Valdés E M, Martín-Mateos M J, Ayuso-Yuste M C. Preservation of white wine pomace by high hydrostatic pressure. Heliyon, 2023; 9（11）: e21199.

[13] Lee H S, Lee H J, Yu H J, Ju D W, Kim Y, Kim C T, et al. A comparison between high hydrostatic pressure extraction and heat extraction of ginsenosides from ginseng （Panax ginseng CA Meyer）. Journal of the Science of Food and Agriculture, 2011; 91（8）: 1466–1473.

[14] Borah M S, Bhagya Raj G V S, Tiwari A, Dash K K. Effect of intermittent microwave convective drying on quality characteristics of persimmon fruit. Journal of Agriculture and Food Research, 2023; 14: 100816.

[15] Pinela J, Barreira J C, Barros L, Verde S C, Antonio A L, Carvalho A M, et al. Suitability of gamma irradiation for preserving fresh-cut watercress quality during cold storage. Food Chemistry, 2016; 206: 50–58.

[16] Cao J K, Jiang W B, Zhao Y M. Experiment guidance of postharvest physiology and biochemistry of fruits and vegetables. Beijing: China Light Industry Press. 2007; 135p.

[17] Cai S Y, Zhang Z Q, Wang J L, Fu Y, Zhang Z K, Khan M R, et al. Effect of exogenous melatonin on postharvest storage quality of passion fruit through antioxidant metabolism. LWT-Food Science and Technology, 2024; 194: 115835.

[18] Wei J. Study on passive air conditioning preservation and quality change of fresh-cut cistanche. Master dissertation. Inner Mongolia: Inner Mongolia Agricultural University, 2022. doi: 10.27229/d.cnki.gnmnu.2022.001327.

[19] Shu P, Li Y J, Xiang L T, Sheng J P, Shen L. Ethylene enhances tolerance to chilling stress in tomato fruit partially through the synergistic regulation between antioxidant enzymes and ATP synthases. Postharvest Biology and Technology, 2022; 193: 112065.

[20] He Q, Jiang Y Q, Huang C Y, Zhang L J, Hou L D, Yao F J, et al. Molecular mechanism of delayed development by interfering RNA targeting the phenylalanine ammonia lyase gene （pal1） in Pleurotus ostreatus. Journal of Integrative Agriculture, 2025; 24（4）: 1477–1488.

[21] Gonzalez M E, Jernstedt J A, Slaughter D C, Barrett D M. Influence of cell integrity on textural properties of raw, high pressure, and thermally processed onions. Journal of Food Science, 2010; 75（7）: E409–E416.

[22] Zhou C L, Liu W, Yuan C, Zhao J, Li Q H. Effects of high hydrostatic pressure processing on microbial inactivation and quality of fresh-cut pumpkin. Transactions of the CSAM, 2014; 45（6）: 227–236. （in Chinese）

[23] Peng J, Lu J H, Zhu J N, Li D J, Li L, Yu Y S, et al. Effect of high hydrostatic pressure pretreatment on physicochemical properties, bioactive components and antioxidant activities of dehydrated yam slices. LWT-Food Science and Technology, 2024; 198: 115954.

[24] Gonzalez M E, Barrett D M, Mccarthy M J, Vergeldt F J, Gerkema E, Matser A M, et al. 1H‐NMR study of the impact of high pressure and thermal processing on cell membrane integrity of onions. Journal of Food Science, 2010; 75（7）: E417–E425.

[25] Tejada-Ortigoza V, García-Amezquita L E, Serna-Saldívar S O, Welti-Chanes J. The dietary fiber profile of fruit peels and functionality modifications induced by high hydrostatic pressure treatments. Food Science and Technology International, 2017; 23（5）: 396–402.

[26] Yang Z X, Duan X K, Yang J Y, Wang H D, Liu F X, Xu X Y, et al. Effects of high hydrostatic pressure and thermal treatment on texture properties of pickled kohlrabi. LWT, 2022; 57: 113078.

[27] Liang D C. A salutary role of reactive oxygen species in intercellular tunnel-mediated communication. Frontiers in Cell and Developmental Biology, 2018; 6: 2.

[28] Denoya G I, Vaudagna S R, Polenta G. Effect of high-pressure processing and vacuum packaging on the preservation of fresh-cut peaches. LWT - Food Science and Technology, 2015; 62（1）: 801–806.

[29] Yıldız H, Baysal T. Effects of alternative current heating treatment on Aspergillus niger, pectin methylesterase and pectin content in tomato. Journal of Food Engineering, 2006; 75（3）: 327–332.

[30] Hu K, Peng D, Wang L, Liu H, Xie B J, Sun Z D. Effect of mild high hydrostatic pressure treatments on physiological and physicochemical characteristics and carotenoid biosynthesis in postharvest mango. Postharvest Biology and Technology, 2021; 72: 111381.

[31] Ganzle M, Liu Y. Mechanisms of pressure-mediated cell death and injury in Escherichia coli: From fundamentals to food applications. Frontiers in Microbiology, 2015; 6: 599.

[32] Feng H H, Yi J Y, Bi J F, Zhou L Y, Li J. Effect of high hydrostatic pressure processing on the physiological characteristics of green asparagus. Modern Food Science and Technology, 2017; 33（6）: 189–194. （in Chinese）

[33] Sun H Y, Ma J, Hao D Q, Jin W G, Li X S. Effect of ultra-high-pressure treatment on preservation of fresh-cut gastrodia elata Bl. Storage and Process, 2019; 19（5）: 53–58, 65. （in Chinese）

[34] Li M L, Lin H T, Chen Y Z, Chen Y H, Lin Y F, Fan Z Q, et al. The role of energy transport system in pericarp browning of harvested longan fruit. Postharvest Biology and Technology, 2024; 207: 112619.

[35] Chen C Y, Peng X, Chen J Y, Gan Z, Wan C P. Mitigating effects of chitosan coating on postharvest senescence and energy depletion of harvested pummelo fruit response to granulation stress. Food Chemistry, 2021; 348: 129113.

[36] Jacobo-Velázquez D A, Del Rosario Cuéllar-Villarreal M, Welti-Chanes J, Cisneros-Zevallos L, Ramos-Parra P A, Hernández-Brenes C. Nonthermal processing technologies as elicitors to induce the biosynthesis and accumulation of nutraceuticals in plant foods. Trends in Food Science & Technology, 2017; 60: 80–87.

[37] Wang L Y, Fu H J, Tan Q, Wu S Y, Wang Y, Peng X L. Preservation effects of chitosan/polyvinyl alcohol/clove essential oil antifungal film on yellow peaches: Physicochemical properties and fruit quality assessment. Food Control, 2024; 164: 110582.

[38] Lin D S, Shuai L, Liu Y F, Pan Z T, Cai W, Yin F L, et al. Effects of high-pressure process treatment on enzymatic browning and related gene expression of fresh-cut lettuce. Food and Fermentation Industries, 2023; 49（17）: 23–30. （in Chinese）

[39] Niu L, Liao W. Hydrogen peroxide signaling in plant development and abiotic responses: crosstalk with nitric oxide and calcium. Frontiers in Plant Science, 2016; 7: 177967.

[40] Ortega V G, Ramírez J A, Velázquez G, Tovar B, Mata M, Montalvo E. Effect of high hydrostatic pressure on antioxidant content of ‘Ataulfo’ mango during postharvest maturation. Food Science and Technology, 2013; 33: 561–568.

[41] Lin Y X, Lin H T, Lin M S, Chen Y H, Wang H, Fan Z Q, et al. Hydrogen peroxide reduced ATPase activity and the levels of ATP, ADP, and energy charge and its association with pulp breakdown occurrence of longan fruit during storage. Food Chemistry, 2020; 311: 126008.

[42] Smetanska I. Production of secondary metabolites using plant cell cultures. In: Ulber S （Ed.）. Advances in Biochemical Engineering/Biotechnology. 2008; pp.187–228.

[43] Abdulhafiz F, Mohammed A, Reduan M F H, Kari Z A, Wei L S, Goh K W. Plant cell culture technologies: A promising alternative to produce high-value secondary metabolites. Arabian Journal of Chemistry, 2022; 15（11）: 104161.

[44] Viacava F, Ortega-Hernández E, Welti-Chanes J, Cisneros-Zevallos L, Jacobo-Velázquez D A. Using high hydrostatic pressure processing come-up time as an innovative tool to induce the biosynthesis of free and bound phenolics in whole carrots. Food and Bioprocess Technology, 2020; 13（10）: 1717–1727.

[45] Jacobo-Velázquez D A, González-Agüero M, Cisneros-Zevallos L. Cross-talk between signaling pathways: The link between plant secondary metabolite production and wounding stress response. Scientific Reports, 2015; 5: 8608.

[46] Zhang X J. Effect of high hydrostatic pressure on quality and microorganism of fresh cut lettuce and its mechanism. PhD dissertation. Beijing: Chinese Academy of Agricultural Sciences, 2012. （in Chinese）

[47] Berno N D, Tezotto-Uliana J V, Dos Santos Dias C T, Kluge R A. Storage temperature and type of cut affect the biochemical and physiological characteristics of fresh-cut purple onions. Postharvest Biology and Technology, 2014; 93: 91–96.

[48] Azeem M, Rehman M A U. Effect of soaking solution and high hydrostatic pressure on in vitro digestibility of β-carotene in gluten free sweet potato noodles. Innovative Food Science & Emerging Technologies, 2024; 94: 103677.

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Published

2026-05-21

How to Cite

(1)
Bao, T.; Li, Q.; Gao, J.; Dong, T.; Eerdunbayaer; Song, L.; Yun, X.; Cheng, P. High Hydrostatic Pressure As a Novel Strategy for the Simultaneous Preservation and Enhancement of Phenylethanol Glycosides in Fresh-Cut Cistanche Deserticola. Int J Agric & Biol Eng 2026, 19, 310-319.

Issue

Vol. 19 No. 2 (2026): IJABE

Section

Agro-product and Food Processing Systems

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